ACROBiosystems/Human Fc gamma RIIIA / CD16a (V176) Protein, His Tag (SPR & BLI verified)/1mg (500ug X 2)/CD8,ACROBiosystems,,ACROBiosystems,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：ACROBiosystems/Human Fc gamma RIIIA / CD16a (V176) Protein, His Tag (SPR & BLI verified)/1mg (500ug X 2)/CD8

产品编号：CD8

市场价：¥36000.00

场地：美国(厂家直采)

产品分类：蛋白类>天然蛋白>球蛋白>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$1800.00

品牌： ACROBiosystems

公司：ACROBiosystems

公司分类：

商品介绍

Synonym
FCGR3A,CD16A,FCG3,FCGR3,IGFR3
Source
Human CD16a (V176), His Tag (SPR& BLI verified) (CD8-H52H4) is expressed from human 293 cells (HEK293). It contains AA Gly 17 - Gln 208 (Accession # P08637-1).
Predicted N-terminus: Gly 17
Request for sequence
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 23.7 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>95% as determined by reduced SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
1. -20°C to -70°C for 12 months in lyophilized state;
2. -70^°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE

Human CD16a (V176), His Tag (SPR& BLI verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-SPR

ImmobilizedHuman CD16a (V176), His Tag (SPR& BLI verified) (Cat. No.CD8-H52H4) on CM5 Chip via anti-His antibody, can bindHerceptinwith anaffinity constant of 163 nM as determined in SPR assay (BiacoreT200) (QC tested).

Protocol

Report

Bioactivity-BLI

Loaded Human CD16a (V176), His Tag (SPR& BLI verified) (Cat. No. CD8-H52H4) on HIS1K Biosensor, can bind Herceptin with an affinity constant of 0.447 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Loaded Herceptin on Protein A Biosensor, can bind Human CD16a (V176), His Tag (SPR & BLI verified) (Cat. No. CD8-H52H4) with an affinity constant of 0.37 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Loaded Herceptin on FAB2G Biosensor, can bind Human CD16a (V176), His Tag (SPR & BLI verified) (Cat. No. CD8-H52H4) with an affinity constant of 0.31 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Citations
- Charge variant analysis of proposed biosimilar to Trastuzumab
  Authors: Dakshinamurthy P, et al.
  Journal: Biologicals 2017
  Application: SPR
  Request for Full-text
Background
CD16 is a low affinity Fc receptor, and has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b). These receptors bind to the Fc portion of IgG antibodies. CD16 encoded by two different highly homologous genes in a cell type-specific manner.CD16 is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages.CD16a antigen is also known as Low affinity immunoglobulin gamma Fc region receptor III-A, Fc-gamma RIII-alpha. CD16b is a low-affinity, GPI-linked receptor expressed by neutrophils and eosinophils, whereas CD16a is an intermediate affinity polypeptide-anchored transmembrane glycoprotein expressed natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placentaltrophoblasts.CD16a is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibodydependent cytotoxicity and clearance of immune complexes. Aberrant expression or mutations of CD16a is implicated in susceptibility to recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia.
References
- (1)Webster NL., et al., 2006, J Leukoc Biol. 79: 294-302.
- (2)Nagarajan S., et al., 1995, J Biol Chem. 270: 25762-70.
- (3)Ravetch JV., 1989, J.Exp.Med.170:481.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

品牌介绍

AcroBiosystems是专注于生物药开发过程的蛋白技术和产品的全球化品牌，目前位于美国特拉华州的纽瓦克市，是一家专业研发、生产、销售蛋白相关分子生物学试剂的高科技公司。AcroBiosystems从成立之初至今一直致力于提供在药物研发过程中所需的高质量的靶点蛋白，满足市场对大批量、高批间*性的蛋白试剂的需求，产品和服务面向全球制药公司、生物技术公司及科研院所。产品包括免疫检查点蛋白、生物素标记蛋白、Fc受体蛋白、CAR-T靶点蛋白等，基于AviTag^TM标记技术和化学标记技术的生物素标记蛋白系列被广泛应用于Biacore，AlphaLisa，MSD等平台。这些产品受到了学术界的*好评。

CD Feed X Supplement Plus, Liquid No.CF-1116

产品介绍
品牌：acrobiosystems
规格：500ml
产品描述：CD细胞的生长的补充液是化学定义的，能显著提高细胞密度和延长细胞活力，从而增加靶蛋白的表达，使用剂量为5-10%（V/V）。与CD 293 TGE培养基配合使用，BPM-BPfectin和293-Expression MAX-1可提高蛋白表达量。
产品特性：① 显著提高生产率
② 无动物源、无蛋白质、无血清和化学成分
储存条件：2℃-8℃，避光。
产品参数：

部分产品的详细列表：AcroBiosystems授权代理

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