ACROBiosystems/Human Fc gamma RIIIB / CD16b (NA2) Protein, His Tag (SPR & BLI verified)/1mg (500ug × 2)/CDB,ACROBiosystems,,ACROBiosystems,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：ACROBiosystems/Human Fc gamma RIIIB / CD16b (NA2) Protein, His Tag (SPR & BLI verified)/1mg (500ug × 2)/CDB

产品编号：CDB

市场价：¥39600.00

场地：美国(厂家直采)

产品分类：蛋白类>天然蛋白>白蛋白>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$1980.00

品牌： ACROBiosystems

公司：ACROBiosystems

公司分类：

商品介绍

Synonym
Fc gamma RIIIB,CD16b (NA2),FCGR3B,CD16B,FCG3B,FCGR3,FCG3,IGFR3
Source
Human CD16b (NA2), His Tag (SPR & BLI verified) (CDB-H5222) is expressed from human 293 cells (HEK293). It contains AA Gly 17 - Ser 200 (Accession # O75015-1).The NA1 form of the CD16b differ with the NA2 form of CD16b in AA36, 65, 82, and 106. The NA1 form carries R36, N65, D82, and V106, while the NA2 form carries S36, S65, N82, and I106.
Predicted N-terminus: Gly 17
Request for sequence
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 22.7 kDa. The protein migrates as 38-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
1. -20°C to -70°C for 12 months in lyophilized state;
2. -70^°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE

Human CD16b (NA2), His Tag (SPR & BLI verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Request for HPLC data

Bioactivity-SPR

Immobilized Human CD16b (NA2), His Tag (SPR & BLI verified) (Cat. No. CDB-H5222) on CM5 Chip via anti-His antibody, can bind Rituximab with an affinity constant of 2.88 μM as determined in a SPR assay (Biacore T200) (QC tested).

Protocol

Bioactivity-BLI

Loaded Human CD16b (NA2), His Tag (SPR & BLI verified) (Cat. No. CDB-H5222) on HIS1K Biosensor, can bind Rituximab with an affinity constant of 7.1 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Citations
- Control of therapeutic IgG antibodies galactosylation during cultivation process and its impact on IgG1/FcγR interaction and ADCC activity
  Authors: Simonov V, et al
  Journal: Biologicals 43480
  Application: SPR
  Request for Full-text
Background
CD16 is a low affinity Fc receptor, and has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b). These receptors bind to the Fc portion of IgG antibodies. CD16 encoded by two different highly homologous genes in a cell type-specific manner.CD16 is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages.CD16B is also kown as FCGR3B and FCG3B, is expressed specifically by polymorphonuclear leukocytes (neutrophils) and stimulated eosinophils. CD16B is the low affinity receptor for the Fc region of immunoglobulins gamma. FCGR3B binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, FCG3B is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. CD16B may serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.
References
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

品牌介绍

AcroBiosystems是专注于生物药开发过程的蛋白技术和产品的全球化品牌，目前位于美国特拉华州的纽瓦克市，是一家专业研发、生产、销售蛋白相关分子生物学试剂的高科技公司。AcroBiosystems从成立之初至今一直致力于提供在药物研发过程中所需的高质量的靶点蛋白，满足市场对大批量、高批间*性的蛋白试剂的需求，产品和服务面向全球制药公司、生物技术公司及科研院所。产品包括免疫检查点蛋白、生物素标记蛋白、Fc受体蛋白、CAR-T靶点蛋白等，基于AviTag^TM标记技术和化学标记技术的生物素标记蛋白系列被广泛应用于Biacore，AlphaLisa，MSD等平台。这些产品受到了学术界的*好评。

CD Feed X Supplement Plus, Liquid No.CF-1116

产品介绍
品牌：acrobiosystems
规格：500ml
产品描述：CD细胞的生长的补充液是化学定义的，能显著提高细胞密度和延长细胞活力，从而增加靶蛋白的表达，使用剂量为5-10%（V/V）。与CD 293 TGE培养基配合使用，BPM-BPfectin和293-Expression MAX-1可提高蛋白表达量。
产品特性：① 显著提高生产率
② 无动物源、无蛋白质、无血清和化学成分
储存条件：2℃-8℃，避光。
产品参数：

部分产品的详细列表：AcroBiosystems授权代理

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