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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
ACROBiosystems/Biotinylated Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control/200ug/DNP
产品编号:DNP
市  场 价:¥25000.00
场      地:美国(厂家直采)
产品分类: 抗体类>一抗>多克隆抗体>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$1250.00
品      牌: ACROBiosystems
公      司:ACROBiosystems
公司分类:
ACROBiosystems/Biotinylated Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control/200ug/DNP
商品介绍
  • Source
    Dinitrophenyl (DNP) is a hapten that is not normally expressed in the target tissue. Therefore, anti-DNP antibody has no relevant specificity to a target antigen, and can be used as isotype control antibody to differentiate non-specific background signal from specific antibody signal in various in vitro and in vivo studies. Biotinylated Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control was purified from HEK293 cell culture. It is the Biotin labeled form ofMonoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control (Cat. No. DNP-M1).
  • Isotype
    Mouse IgG1/kappa
  • Specificity
    Specifically reacts with DNP (Dinitrophenyl) and DNP conjugated proteins.
  • Application
    This antibody is suitable for use as a non-targeting isotype control in various in vitro and in vivo studies. It can also be used as a negative control in various applications such as ELISA, Western blot, immunofluorescence, immunohistochemistry, immunoprecipitation, and flow cytometry. Each laboratory should determine an optimum working titer for use in its particular application.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control (Cat. No. DNP-BM1) SDS-PAGE gel

Biotinylated Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

2e5 of Anti-CD19 CAR-293 cells were stained with a series of concentrations of Biotinylated Anti-DNP Antibody, Mouse IgG1 (Cat. No. DNP-BM1), washed and then followed by PE-SA and analyzed with FACS (QC tested).

Protocol
FACS assay shows that recombinant Biotinylated FACS

5e5 of PBMCs were stained with a series of concentrations of Biotinylated Anti-DNP Antibody, Mouse IgG1 (Cat. No. DNP-BM1), washed and then followed by PE-SA and analyzed with FACS (QC tested).

Protocol
FACS assay shows that recombinant Biotinylated FACS

2e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1 μg/mL of Biotinylated Anti-DNP Antibody, Mouse IgG1 (Cat. No. DNP-BM1) and positive control respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).

Protocol
  • Background
    A hapten is a small molecule that can elicit an immune response only when conjugated with a large carrier such as a protein. Typical haptens include drugs, urushiol, quinone, steroids, etc. Peptides and non-protein antigens usually need conjugating to a carrier protein (such as BSA (bovine serum albumin) or KLH (keyhole limpet hemocyanin) to become good immunogens). Additionally, haptens should be administered with an adjuvant to ensure a high quality immune response. It is important that the hapten design (preserving greatly the chemical structure and spatial conformation of target compound), selection of the appropriate carrier protein and the conjugation method are key conditions for the desired specificity anti-hapten antibodies. We design anti-hapten antibodies based on the HaptenDB information.
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

品牌介绍

AcroBiosystems是专注于生物药开发过程的蛋白技术和产品的全球化品牌,目前位于美国特拉华州的纽瓦克市,是一家专业研发、生产、销售蛋白相关分子生物学试剂的高科技公司。AcroBiosystems从成立之初至今一直致力于提供在药物研发过程中所需的高质量的靶点蛋白,满足市场对大批量、高批间*性的蛋白试剂的需求,产品和服务面向全球制药公司、生物技术公司及科研院所。产品包括免疫检查点蛋白、生物素标记蛋白、Fc受体蛋白、CAR-T靶点蛋白等,基于AviTagTM标记技术和化学标记技术的生物素标记蛋白系列被广泛应用于Biacore,AlphaLisa,MSD等平台。这些产品受到了学术界的*好评。

 

CD Feed X Supplement Plus, Liquid  No.CF-1116

产品介绍
品牌:acrobiosystems
规格:500ml
产品描述:CD细胞的生长的补充液是化学定义的,能显著提高细胞密度和延长细胞活力,从而增加靶蛋白的表达,使用剂量为5-10%(V/V)。与CD 293 TGE培养基配合使用,BPM-BPfectin和293-Expression MAX-1可提高蛋白表达量。
产品特性:① 显著提高生产率
          ② 无动物源、无蛋白质、无血清和化学成分
储存条件:2℃-8℃,避光。
产品参数:

 

部分产品的详细列表:AcroBiosystems授权代理

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