ACROBiosystems/Mouse Fc gamma RIV / CD16-2 Protein, His Tag/1mg/FC4,ACROBiosystems,,ACROBiosystems,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：ACROBiosystems/Mouse Fc gamma RIV / CD16-2 Protein, His Tag/1mg/FC4

产品编号：FC4

市场价：¥46200.00

场地：美国(厂家直采)

产品分类：蛋白类>重组蛋白>细菌抗原>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$2310.00

品牌： ACROBiosystems

公司：ACROBiosystems

公司分类：

商品介绍

Synonym
Fc gamma RIV,CD16-2,Fcgr4
Source
Mouse CD16-2, His Tag (FC4-M52H3) is expressed from human 293 cells (HEK293). It contains AA Gly 21 - Gln 203 (Accession # A0A0B4J1G0-1).
Predicted N-terminus: Gly 21
Request for sequence
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 22.8 kDa. The protein migrates as 28-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
1. -20°C to -70°C for 12 months in lyophilized state;
2. -70^°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE

Mouse CD16-2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-SPR

Captured Mouse CD16-2, His Tag (Cat. No. FC4-M52H3) on NTA-Series S sensor chip can bind OKT3 with an affinity constant of 154 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

Bioactivity-BLI

Loaded Mouse CD16-2, His Tag (Cat. No. FC4-M52H3) on NTA Biosensor, can bind OKT3 with an affinity constant of 140 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Loaded Mouse CD16-2, His Tag (Cat. No. FC4-M52H3) on NTA Biosensor, can bind Herceptin with an affinity constant of 122 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Citations
- CD38 ecto-enzyme in immune cells is induced during aging and regulates NAD + and NMN levels
  Authors: Chini CCS, Peclat TR, Warner GM, et al.
  Journal: Nature metabolism 2020
  Application: Binding Assay
  Request for Full-text
Background
FcgR4(Low affinity immunoglobulin gamma Fc region receptor IV) is also known as CD16-2, FcgammaRIV, receptor for the Fc region of immunoglobulin gamma . Also acts as a receptor for the Fc region of immunoglobulin epsilon . Binds with intermediate affinity to both IgG2a and IgG2b . Does not display binding to IgG1 or IgG3. Plays a role in promoting bone resorption by enhancing osteoclast differentiation following binding to IgG2a. Binds with low affinity to both the a and b allotypes of IgE. Has also been shown to bind to IgE allotype a only but not to allotype b. Binding to IgE promotes macrophage-mediated phagocytosis, antigen presentation to T cells, production of proinflammatory cytokines and the late phase of cutaneous allergic reactions
References
- (1)Hirano M, et al. 2007. Nat Immunol. 8(7):762-71.
- (2)Mancardi DA. 2008. J Clin Invest. 118(11):3738-50.
- (3)Nimmerjahn F. 2005. Immunity. 23(1):41-51.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

品牌介绍

AcroBiosystems是专注于生物药开发过程的蛋白技术和产品的全球化品牌，目前位于美国特拉华州的纽瓦克市，是一家专业研发、生产、销售蛋白相关分子生物学试剂的高科技公司。AcroBiosystems从成立之初至今一直致力于提供在药物研发过程中所需的高质量的靶点蛋白，满足市场对大批量、高批间*性的蛋白试剂的需求，产品和服务面向全球制药公司、生物技术公司及科研院所。产品包括免疫检查点蛋白、生物素标记蛋白、Fc受体蛋白、CAR-T靶点蛋白等，基于AviTag^TM标记技术和化学标记技术的生物素标记蛋白系列被广泛应用于Biacore，AlphaLisa，MSD等平台。这些产品受到了学术界的*好评。

CD Feed X Supplement Plus, Liquid No.CF-1116

产品介绍
品牌：acrobiosystems
规格：500ml
产品描述：CD细胞的生长的补充液是化学定义的，能显著提高细胞密度和延长细胞活力，从而增加靶蛋白的表达，使用剂量为5-10%（V/V）。与CD 293 TGE培养基配合使用，BPM-BPfectin和293-Expression MAX-1可提高蛋白表达量。
产品特性：① 显著提高生产率
② 无动物源、无蛋白质、无血清和化学成分
储存条件：2℃-8℃，避光。
产品参数：

部分产品的详细列表：AcroBiosystems授权代理

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