ACROBiosystems/Human IgG1 Fc Protein, Tag Free/200ug/FCC,ACROBiosystems,,ACROBiosystems,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：ACROBiosystems/Human IgG1 Fc Protein, Tag Free/200ug/FCC

产品编号：FCC

市场价：¥12000.00

场地：美国(厂家直采)

产品分类：仪器类>影像显示>凝胶成像仪>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$600.00

品牌： ACROBiosystems

公司：ACROBiosystems

公司分类：

商品介绍

Synonym
IgG1
Source
Human IgG Fc, Tag Free (FCC-H5214) is expressed from human 293 cells (HEK293). It contains AA Glu 99 - Lys 330 (Accession # P01857-1).
Predicted N-terminus: Glu 99
Request for sequence
Molecular Characterization
This protein carries no "tag".
The protein has a calculated MW of 26.1 kDa. The protein migrates as 32-34 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehaloseis added as protectant before lyophilization.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
1. -20°C to -70°C for 12 months in lyophilized state;
2. -70^°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE

Human IgG Fc, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA

Human IgG Fc, Tag FreeHuman IgG Fc, Tag Free (Cat. No. FCC-H5214) ELISA bioactivity

Immobilized Human CD64, His Tag (Cat. No. FCA-H52H2) at 10μg/mL (100 μL/well) can bind Human IgG Fc, Tag Free (Cat. No. FCC-H5214) with a linear range of 7-41 ng/mL (QC tested).

Protocol

Bioactivity-SPR

Human FCGRT&B2M Heterodimer Protein, His Tag (SPR & BLI verified) (Cat. No. FCN-H52W7) captured on CM5 Chip via anti-His antibody can bind Human IgG1 Fc, Tag Free (Cat. No. FCC-H5214) with an affinity constant of 0.957 μM as determined in SPR assay (Biacore 8K) (Routinely tested).

Protocol

Citations
- Site-Specific Crosslinking of Antibodies
  Authors: B Agnew, et al
  Journal: US20190177438A1 43629
  Application: Fluorescent labeled
  Request for Full-text
- Compositions and methods for inhibiting WNT signaling
  Authors: M Dong, et al
  Journal: 20190031734A1 43496
  Application: BLI
  Request for Full-text
- Impact of payload hydrophobicity on stability of antibody-drug-conjugates
  Authors: Buecheler JW, et al.
  Journal: Mol Pharm 2018
  Application: BLI
  Request for Full-text
- Single chain Fc-dimer-human growth hormone fusion protein for improved drug delivery
  Authors: Li Zhou, et al.
  Journal: Biomaterials 2016
  Application: As a control in Cell-based assay
  Request for Full-text
- Frizzled proteins are colonic epithelial receptors for C. difficile toxin B
  Authors: Tao L, et al.
  Journal: Nature 2016
  Application: As a control in BLI assay
  Request for Full-text
- Multifunctional RNA nanoparticles
  Authors: Kirill A. Afonin, et al.
  Journal: Nano Lett 2014
  Application: As a control in Cell-based assay
  Request for Full-text
Background
Crystallizable fragments composed of the carboxy-terminal halves of both IMMUNOGLOBULIN HEAVY CHAINS linked to each other by disulfide bonds. Fc fragments contain the carboxy-terminal parts of the heavy chain constant regions that are responsible for the effector functions of an immunoglobulin (COMPLEMENT fixation, binding to the cell membrane via FC RECEPTORS, and placental transport). IgG1 Fc was reported has a novel role as a potential anti-inflammatory drug for treatment of human autoimmune diseases.
References
- (1)Anthony RM, et al., 2010, J Clin Immunol, 30 Suppl 1:S9-14.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

品牌介绍

AcroBiosystems是专注于生物药开发过程的蛋白技术和产品的全球化品牌，目前位于美国特拉华州的纽瓦克市，是一家专业研发、生产、销售蛋白相关分子生物学试剂的高科技公司。AcroBiosystems从成立之初至今一直致力于提供在药物研发过程中所需的高质量的靶点蛋白，满足市场对大批量、高批间*性的蛋白试剂的需求，产品和服务面向全球制药公司、生物技术公司及科研院所。产品包括免疫检查点蛋白、生物素标记蛋白、Fc受体蛋白、CAR-T靶点蛋白等，基于AviTag^TM标记技术和化学标记技术的生物素标记蛋白系列被广泛应用于Biacore，AlphaLisa，MSD等平台。这些产品受到了学术界的*好评。

CD Feed X Supplement Plus, Liquid No.CF-1116

产品介绍
品牌：acrobiosystems
规格：500ml
产品描述：CD细胞的生长的补充液是化学定义的，能显著提高细胞密度和延长细胞活力，从而增加靶蛋白的表达，使用剂量为5-10%（V/V）。与CD 293 TGE培养基配合使用，BPM-BPfectin和293-Expression MAX-1可提高蛋白表达量。
产品特性：① 显著提高生产率
② 无动物源、无蛋白质、无血清和化学成分
储存条件：2℃-8℃，避光。
产品参数：

部分产品的详细列表：AcroBiosystems授权代理

货号	产品	规格	价格（美元）
BAF-HF268	FITC-Labeled Human BAFF / TNFSF13B / CD257 Protein, Fc Tag	25ug	$290
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BCA-HF2H1	FITC-Labeled Human BCMA, His Tag	25ug	$290