- SynonymIgG2A
- SourceBiotinylated Mouse IgG2a Fc, Avitag (IGA-M8210) is expressed from human 293 cells (HEK293). It contains AA Glu 98 - Lys 330 (Accession # P01863).Predicted N-terminus: Glu 98Request for sequence
- Molecular Characterization
This protein carries an Avi tag at the C-terminus.
The protein has a calculated MW of 28.2 kDa. The protein migrates as 36 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- BiotinylationBiotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
- Biotin:Protein RatioThe biotin to protein ratio is 0.7-1 as determined by the HABA assay.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Mouse IgG2a Fc, Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Recombinant Protein A at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse IgG2a Fc, Avitag (Cat. No. IGA-M8210) with a linear range of 0.8-13 ng/mL (QC tested).
Immobilized Mouse CD16-2, His Tag (Cat. No. FC4-M52H3) at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse IgG2a Fc, Avitag (Cat. No. IGA-M8210) with a linear range of 0.01-0.313 μg/mL (Routinely tested).
- BackgroundImmunoglobulin G2 (IgG2) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. There are two members of IgG2: IgG2a and IgG2b. It was found that IgG2a was superior to IgG1 in activating complement. The glycosylation of the circulating immunoglobulin-γ (IgG) antibody molecules changes in rheumatoid arthritis.
- References
- (1)Sikorav J.-L., et al., 1980, Nucleic Acids Res. 8:3143-3155.
- (2)Ollo R., et al., 1981, Proc. Natl. Acad. Sci. U.S.A. 78:2442-2446.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
AcroBiosystems是专注于生物药开发过程的蛋白技术和产品的全球化品牌，目前位于美国特拉华州的纽瓦克市，是一家专业研发、生产、销售蛋白相关分子生物学试剂的高科技公司。AcroBiosystems从成立之初至今一直致力于提供在药物研发过程中所需的高质量的靶点蛋白，满足市场对大批量、高批间*性的蛋白试剂的需求，产品和服务面向全球制药公司、生物技术公司及科研院所。产品包括免疫检查点蛋白、生物素标记蛋白、Fc受体蛋白、CAR-T靶点蛋白等，基于AviTagTM标记技术和化学标记技术的生物素标记蛋白系列被广泛应用于Biacore，AlphaLisa，MSD等平台。这些产品受到了学术界的*好评。
CD Feed X Supplement Plus, Liquid No.CF-1116
产品介绍
品牌：acrobiosystems
规格：500ml
产品描述：CD细胞的生长的补充液是化学定义的，能显著提高细胞密度和延长细胞活力，从而增加靶蛋白的表达，使用剂量为5-10%（V/V）。与CD 293 TGE培养基配合使用，BPM-BPfectin和293-Expression MAX-1可提高蛋白表达量。
产品特性：① 显著提高生产率
② 无动物源、无蛋白质、无血清和化学成分
储存条件：2℃-8℃，避光。
产品参数：
部分产品的详细列表：AcroBiosystems授权代理
货号 | 产品 | 规格 | 价格（美元） |
BAF-HF268 | FITC-Labeled Human BAFF / TNFSF13B / CD257 Protein, Fc Tag | 25ug | $290 |
BCA-HF254 | FITC-Labeled Human BCMA, Fc Tag | 25ug | $290 |
BCA-HF2H1 | FITC-Labeled Human BCMA, His Tag | 25ug | $290 |
![ACROBiosystems/Influenza A[A/swine/Jiangsu/J004/2018(H1N1)] Hemagglutinin (HA) Protein, His Tag/1mg/HEN](images/no_picture.gif)
暂无问答
本网站将在规定时间内给予删除等相关处理。
