ACROBiosystems/Biotinylated Human OX40 / TNFRSF4 / CD134 Protein, Avitag™,His Tag (MALS verified) (recommended for biopanning)/25ug/TN4,ACROBiosystems,,ACROBiosystems,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：ACROBiosystems/Biotinylated Human OX40 / TNFRSF4 / CD134 Protein, Avitag™,His Tag (MALS verified) (recommended for biopanning)/25ug/TN4

产品编号：TN4

市场价：¥48800.00

场地：美国(厂家直采)

产品分类：蛋白类>重组蛋白>细菌抗原>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$2440.00

品牌： ACROBiosystems

公司：ACROBiosystems

公司分类：

商品介绍

Synonym
TNFRSF4,OX40,CD134,OX40L receptor,ACT35,TXGP1L
Source
Biotinylated Human OX40, Avitag,His Tag (TN4-H82E4) is expressed from human 293 cells (HEK293). It contains AA Leu 29 - Ala 216 (Accession # NP_003318.1).
Predicted N-terminus: Leu 29
Request for sequence
Molecular Characterization
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 22.8 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Biotin:Protein Ratio
The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
1. -20°C to -70°C for 12 months in lyophilized state;
2. -70^°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE

Biotinylated Human OX40, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Biotinylated Human OX40, Avitag,His Tag (Cat. No. ) MALS images

The purity of Biotinylated Human OX40, Avitag,His Tag (Cat. No. TN4-H82E4) was more than 90% and around 35-50 kDa verified by SEC-MALS.

Protocol

Bioactivity-ELISA

Biotinylated Human OX40, Avitag,His TagBiotinylated Human OX40, Avitag,His Tag (Cat. No. TN4-H82E4) ELISA bioactivity

Immobilized Anti-OX40 MAb, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human OX40, Avitag,His Tag (Cat. No. TN4-H82E4) with a linear range of 1-5 ng/mL (QC tested).

Protocol

Immobilized Biotinylated Human OX40, Avitag,His Tag (Cat. No. TN4-H82E4) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Human OX40 Ligand, Fc Tag (Cat. No. OXL-H526x) with a linear range of 0.2-10 ng/mL (Routinely tested).

Protocol

Bioactivity-BLI

Loaded Biotinylated Human OX40, Avitag,His Tag (Cat. No. TN4-H82E4) on SA Biosensor, can bind Anti-OX40 MAb (Human IgG1) with an affinity constant of 0.115 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Loaded Biotinylated Human OX40, Avitag,His Tag (Cat. No. TN4-H82E4) on SA Biosensor, can bind Human OX40 Ligand, His Tag (active trimer) (MALS verified) (Cat. No. OXL-H52Q8) with an affinity constant of 0.127 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Citations
- ANTI-OX40 ANTIBODY AND USE THEREOF
  Authors: A T sun, et al
  Journal: US20200140562A1 2020
  Application: MSD-SET Kinetic Assay
  Request for Full-text
Background
Tumor necrosis factor receptor superfamily member 4 (TNFRSF4) is also known as ACT35 antigen, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor, CD antigen CD134, OX40. OX40 / TNFRSF4 contains four TNFR-Cys repeats. TNFRSF4 is receptor for TNFSF4 / OX40L / GP34 and can interacts with TRAF2, TRAF3 and TRAF5.
References
- (1)Arch R.H., et al., 1998, Mol. Cell. Biol. 18:558-565.
- (2)Kawamata S., et al., 1998, J. Biol. Chem. 273:5808-5814.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

品牌介绍

AcroBiosystems是专注于生物药开发过程的蛋白技术和产品的全球化品牌，目前位于美国特拉华州的纽瓦克市，是一家专业研发、生产、销售蛋白相关分子生物学试剂的高科技公司。AcroBiosystems从成立之初至今一直致力于提供在药物研发过程中所需的高质量的靶点蛋白，满足市场对大批量、高批间*性的蛋白试剂的需求，产品和服务面向全球制药公司、生物技术公司及科研院所。产品包括免疫检查点蛋白、生物素标记蛋白、Fc受体蛋白、CAR-T靶点蛋白等，基于AviTag^TM标记技术和化学标记技术的生物素标记蛋白系列被广泛应用于Biacore，AlphaLisa，MSD等平台。这些产品受到了学术界的*好评。

CD Feed X Supplement Plus, Liquid No.CF-1116

产品介绍
品牌：acrobiosystems
规格：500ml
产品描述：CD细胞的生长的补充液是化学定义的，能显著提高细胞密度和延长细胞活力，从而增加靶蛋白的表达，使用剂量为5-10%（V/V）。与CD 293 TGE培养基配合使用，BPM-BPfectin和293-Expression MAX-1可提高蛋白表达量。
产品特性：① 显著提高生产率
② 无动物源、无蛋白质、无血清和化学成分
储存条件：2℃-8℃，避光。
产品参数：

部分产品的详细列表：AcroBiosystems授权代理

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