ACROBiosystems/Human TRAIL / TNFRSF10 Protein, Mouse IgG2a Fc Tag/100ug/TRL,ACROBiosystems,,ACROBiosystems,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：ACROBiosystems/Human TRAIL / TNFRSF10 Protein, Mouse IgG2a Fc Tag/100ug/TRL

产品编号：TRL

市场价：¥46200.00

场地：美国(厂家直采)

产品分类：细胞相关>细胞株>原代细胞>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$2310.00

品牌： ACROBiosystems

公司：ACROBiosystems

公司分类：

商品介绍

Synonym
Apo-2L,TRAIL,CD253,TNFSF10,Apo-2 ligand
Source
Human TRAIL, Mouse IgG2a Fc Tag (TRL-H5259) is expressed from human 293 cells (HEK293). It contains AA Val 114 - Gly 281 (Accession # P50591-1).
Predicted N-terminus: Glu
Request for sequence
Molecular Characterization
This protein carries a mouse IgG2a Fc tag at the N-terminus.
The protein has a calculated MW of 46.4 kDa. The protein migrates as 47-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.1 EU per μg by the LAL method.
Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
1. -20^°C to -70^°C for 12 months in lyophilized state;
2. -70^°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE

Human TRAIL, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA

Human TRAIL, Mouse IgG2a Fc TagHuman TRAIL, Mouse IgG2a Fc Tag (Cat. No. TRL-H5259) ELISA bioactivity

Immobilized Human Osteoprotegerin, His Tag (Cat. No. TNB-H5220) at 5 μg/mL (100 μL/well) can bind Human TRAIL, Mouse IgG2a Fc Tag (Cat. No. TRL-H5259) with a linear range of 0.8-13 ng/mL (QC tested).

Protocol

Immobilized Human TRAIL R1, His Tag (Cat. No. TR1-H5228) at 5 μg/mL (100 μL/well) can bind Human TRAIL, Mouse IgG2a Fc Tag (Cat. No. TRL-H5259) with a linear range of 2-20 ng/mL (Routinely tested).

Protocol

Immobilized Human TRAIL, Mouse IgG2a Fc Tag (Cat. No. TRL-H5259) at 5 μg/mL (100 μL/well) can bind Human TRAIL R4, Fc Tag (Cat. No. TR4-H5256) with a linear range of 0.01-0.313 μg/mL (Routinely tested).

Protocol

Immobilized Human TRAIL, Mouse IgG2a Fc Tag (Cat. No. TRL-H5259) at 5 μg/mL (100 μL/well) can bind Human TRAIL R2, Fc Tag (Cat. No. TR2-H5255) with a linear range of 0.6-20 ng/mL (Routinely tested).

Protocol

Background
TRAIL, also known asTNFSF10, APO2L, Apo-2L, CD253, TL2, TRAIL, TNLG6A and TNF superfamily member 10. The TRAIL gene as a drug target, induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. TRAIL also binds the receptors DcR1 and DcR2, which do not contain a cytoplasmic domain (DcR1) or contain a truncated death domain (DcR2). DcR1 functions as a TRAIL-neutralizing decoy-receptor. The cytoplasmic domain of DcR2 is functional and activates NFkappaB. In cells expressing DcR2, TRAIL binding therefore activates NFkappaB, leading to transcription of genes known to antagonize the death signaling pathway and/or to promote inflammation.
References
- (1)Song JJ, et al. 2008. Cell Signal. 20(5):892-906.
- (2)Ramamurthy V, et al. 2015. Acta Crystallogr F Struct Biol Commun. 71(Pt 10):1273-81.
- (3)Hymowitz SG, et al. 1999. Mol Cell. 4(4):563-71.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

品牌介绍

AcroBiosystems是专注于生物药开发过程的蛋白技术和产品的全球化品牌，目前位于美国特拉华州的纽瓦克市，是一家专业研发、生产、销售蛋白相关分子生物学试剂的高科技公司。AcroBiosystems从成立之初至今一直致力于提供在药物研发过程中所需的高质量的靶点蛋白，满足市场对大批量、高批间*性的蛋白试剂的需求，产品和服务面向全球制药公司、生物技术公司及科研院所。产品包括免疫检查点蛋白、生物素标记蛋白、Fc受体蛋白、CAR-T靶点蛋白等，基于AviTag^TM标记技术和化学标记技术的生物素标记蛋白系列被广泛应用于Biacore，AlphaLisa，MSD等平台。这些产品受到了学术界的*好评。

CD Feed X Supplement Plus, Liquid No.CF-1116

产品介绍
品牌：acrobiosystems
规格：500ml
产品描述：CD细胞的生长的补充液是化学定义的，能显著提高细胞密度和延长细胞活力，从而增加靶蛋白的表达，使用剂量为5-10%（V/V）。与CD 293 TGE培养基配合使用，BPM-BPfectin和293-Expression MAX-1可提高蛋白表达量。
产品特性：① 显著提高生产率
② 无动物源、无蛋白质、无血清和化学成分
储存条件：2℃-8℃，避光。
产品参数：

部分产品的详细列表：AcroBiosystems授权代理

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